Fig 1. Biovolume of the individual components in biofilms formed by Salmonella Agona on polystyrene after varying periods of development.

¹, propidium iodide; ², fluorescein isothiocyanate isomer I; ³, DiIC₁₈(5) oil, 1,1'-dioctadecyl-3,3,3',3'-tetramethylindodicarbocyanine perchlorate; ⁴, concanavalin A, tetramethylrhodamine conjugate; ⁵, calcofluor white M2R. Bars (mean ± SD) in the graph for the same fluorescent dye without any letter in common are significantly different (P < 0.05). The images correspond to three-dimensional reconstructions obtained from confocal stack images by the IMARIS 9.1 software, virtual projections being included on the right.