Fig 3. Biovolumes of cells and EPS in biofilms formed by Salmonella Agona on polystyrene over the course of 144 hours of incubation at 37°C.

A, cells and extracellular polymeric substances (EPS), expressed as the biovolume (μm³) in the observation field (16,078.2 μm²); B, cells and EPS, expressed as the percentage of biovolume relative to total biovolume of the biofilm; C, live cells, dead cells, proteins, lipids, α-polysaccharides and β-polysaccharides, expressed as the biovolume (μm³) in the observation field (16,078.2 μm²); D, live cells, dead cells, proteins, lipids, α-polysaccharides and β-polysaccharides, expressed as the percentage of biovolume relative to the total biovolume of the biofilm.