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. 2018 Jul 10;13(7):e0200011. doi: 10.1371/journal.pone.0200011

Table 1. Stains and microscopic parameters used in study the cellular and extracellular components of the biofilms formed by Salmonella Agona.

Dye SYTO 9 PI1 FITC2 DiD’oil3 ConA-TMR4 CFW5
Pinhole 44 μm 44 μm 50 μm 56 μm 52 μm 40 μm
Laser Wavelength 488 nm: 0,20% 561 nm: 4,50% 488 nm: 0,20% 640 nm: 2,00% 561 nm: 4,50% 405 nm: 3,00%
Excitation Wavelength 483 nm 305 nm 495 nm 648 nm 552 nm 254 nm
Emission Wavelength 500 nm 617 nm 519 nm 670 nm 578 nm 432 nm
Detection Wavelength 450–560 nm 560–700 nm 400–700 nm 645–700 nm 400–700 nm 400–560 nm
Imaging Device LSM 800 / Airyscan LSM 800 / GaAsP-Pmt2 LSM 800 / GaAsP-Pmt1 LSM 800 / GaAsP-Pmt2 LSM 800 / GaAsP-Pmt1 LSM 800 / GaAsP-Pmt1
Detector Airyscan GaAsP GaAsP GaAsP GaAsP GaAsP
Detector Gain 700 V 660 V 700 V 800 V 660 V 850 V
Target Live cells Dead cells Proteins Lipids α-polysaccharides β-polysaccharides

1, propidium iodide

2, fluorescein isothiocyanate isomer I

3, DiIC18(5) oil, 1,1'-dioctadecyl-3,3,3',3'-tetramethylindodicarbocyanine perchlorate

4, concanavalin A, tetramethylrhodamine conjugate

5, calcofluor white M2R.