Table 1. Stains and microscopic parameters used in study the cellular and extracellular components of the biofilms formed by Salmonella Agona.

Dye	SYTO 9	PI1	FITC2	DiD’oil3	ConA-TMR4	CFW5
Pinhole	44 μm	44 μm	50 μm	56 μm	52 μm	40 μm
Laser Wavelength	488 nm: 0,20%	561 nm: 4,50%	488 nm: 0,20%	640 nm: 2,00%	561 nm: 4,50%	405 nm: 3,00%
Excitation Wavelength	483 nm	305 nm	495 nm	648 nm	552 nm	254 nm
Emission Wavelength	500 nm	617 nm	519 nm	670 nm	578 nm	432 nm
Detection Wavelength	450–560 nm	560–700 nm	400–700 nm	645–700 nm	400–700 nm	400–560 nm
Imaging Device	LSM 800 / Airyscan	LSM 800 / GaAsP-Pmt2	LSM 800 / GaAsP-Pmt1	LSM 800 / GaAsP-Pmt2	LSM 800 / GaAsP-Pmt1	LSM 800 / GaAsP-Pmt1
Detector	Airyscan	GaAsP	GaAsP	GaAsP	GaAsP	GaAsP
Detector Gain	700 V	660 V	700 V	800 V	660 V	850 V
Target	Live cells	Dead cells	Proteins	Lipids	α-polysaccharides	β-polysaccharides

¹, propidium iodide

², fluorescein isothiocyanate isomer I

³, DiIC₁₈(5) oil, 1,1'-dioctadecyl-3,3,3',3'-tetramethylindodicarbocyanine perchlorate

⁴, concanavalin A, tetramethylrhodamine conjugate

⁵, calcofluor white M2R.