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. 2018 Jul 10;13(7):e0200416. doi: 10.1371/journal.pone.0200416

Fig 6. Inhibition of Notch signalling does not prevent EDEC development.

Fig 6

hPSCs were differentiated into EDECs while treating them with γ-secretase inhibitors. (A) HFF derived cells treated with Compound E (left) or H1 derived cells treated with γ-secretase inhibitor I (right) adopted the EDEC morphology. (B) Immunocytochemistry for CK19, SOX9, ECAD, and OPN. (C,D) qRT-PCR for hepatocyte or cholangiocyte determining genes. Gene expression was normalized to RPS16 and fold change was calculated relative to untreated cells. Mean values of technical triplicates of biological duplicates (C) or of one sample (D) are show.