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. 2018 Jun 27;16(6):e2004425. doi: 10.1371/journal.pbio.2004425

Fig 1. BMP4 expression overlaps with intracellular BMP inhibitor expression and intermediate Smad1/5/8 activation.

Fig 1

(A) HCR shows expression of bmp4, ckip-1, and smurf1 in wild-type HH6 chick embryos. (B) Immunostaining for the NPB marker Pax7 and phosphorylated Smad1/5/8 in HH6 embryos. (C) Line traces of bmp4, ckip-1, and smurf1 expression compared with Pax7 and pSmad1/5/8 staining intensity from the boxed regions in A-B show high bmp4 expression and intermediate pSmad1/5/8 staining in the Pax7+ NPB domain. Underlying data can be found in S1 Data. Scale bars represent 200 μm. BMP, bone morphogenetic protein; CKIP-1, casein kinase interacting protein 1; HCR, hybridization chain reaction; HH6, Hamburger-Hamilton stage 6; NNE, nonneural ectoderm; NP, neural plate; NPB, neural plate border; Pax7, paired box 7; pSmad1/5/8, phospho-Smads 1/5/8; Smurf1, Smad ubiquitin regulatory factor 1.