MASTER-MIX | n=1 | Cat-Nr |
---|---|---|
H2O | 17.5 μl | G-1000–1 250 units |
10x PCR Buffer | 2.5 μl | G-1000–1 250 units |
MgCl2 (25mM) | 2 μl | G-1000–1 250 units |
dNTP (10 mM) | 0.5 μl | G-1000–1 250 units |
Primer IVS II-I (1:10) | 0.3 μl | BIO NEER |
0.3 μl | ||
Prime Taq DNA Polymerase (5 U/μl) | 0.3 μl | G-1000–1 250 units |
DNA | 1 μl |