(A) JQ-EZ-05 structure.
(B) Inhibition of recombinant EZH1 and EZH2 by JQ-EZ-05 in vitro.
(C) Immunoblots of CAKI-2 cells treated with 1 μM JQ-EZ-05 for the indicated number of days or treated for 5 days followed by removal (washout) for 1–3 days.
(D) Heatmap displaying gain (red and green) or loss (blue and black) of histone marks in 2 biological replicates (labeled 1 and 2) of CAKI-2 cells treated with the indicated concentrations of JQ-EZ-05 or DMSO vehicle for either 24 hours (D1) or 1 week (D7).