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. Author manuscript; available in PMC: 2019 Jun 1.
Published in final edited form as: Gastroenterology. 2018 Feb 15;154(8):2209–2221.e14. doi: 10.1053/j.gastro.2018.02.015

Fig. 6. Stiffness potentiates tumor-promoting effects of HSCs through HSC p300.

Fig. 6

A HSC-conditioned media (CMs) were used as stimulants for HT29 proliferation assay. HSC 25.6 kPa CM promoted HT29 proliferation as compared to 0.4 kPa CM. *, p<0.05 by ANOVA, n=5. B. Recombinant CXCL12 dose-dependently promoted HT29 proliferation. *, p<0.05 by ANOVA, n=5. C. The effect of HSC-CM on HT29 proliferation was suppressed by p300 knockdown in HSCs. *, p<0.05 by ANOVA, n=5. D. Serum-starved HT29 cells stimulated with HSC-CM at 37°C for 2 hours were plated in a 96-well plate (30,000 cells/well) and cultured in serum-free DMEM for additional 24 hours. MTS assay revealed that in vitro stimulation with HSC-CM accelerated cell proliferation. *, p<0.05 by t-test, n=6. E. Serum-starved HT29 were pretreated with CM at 37°C for 2 hours and resuspended in CM, and HT29/CM were coinjected into SCID mice subcutaneously. Tumor nodules were isolated and quantitated at day 7. *, P<0.05 by ANOVA, n=7 or 8 per group. F. Tumor lysates were subjected to WB for α-SMA and PECAM-1/CD31. *, P<0.05 by ANOVA, n=3.