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. Author manuscript; available in PMC: 2018 Jul 10.
Published in final edited form as: Nature. 2018 Jan 17;553(7689):521–525. doi: 10.1038/nature25438

Figure 4. Structure of triplex and function of Tri1 N-anchor.

Figure 4

a, Distribution of triplexes in the MCP network. b, Enlarged view of a triplex Tb from outside the capsid. c–f, Detailed structures of triplex Tb (c) and its components Tri1 (d), Tri2A (e) and Tri2B (f). g, Superposition of Tri2A and Tri2B. h, Triplex Tb viewed from inside the capsid showing similar structures among the Tri2A and Tri2B trunk domains and the Tri1 third-wheel domain. i, Tri2A and Tri2B form a dimer with their embracing arms. The dotted circles denote two disulfide bonds between Tri2A and Tri2B (shown expanded in the inset). j, Triplex Tb viewed from inside the capsid, showing that it anchors to the capsid floor by the tripod-shaped Tri1 N-anchor. k, Comparison of viral titres of wild type and Tri1-truncated mutants. Data are mean ± s.e.m. (n = 3 biologically independent samples). l, m, Transmission electron microscopy images of ultrathin sections of cells replicating the wild-type virus (l) or the Tri1Δ N65 mutant (m). Experiments were repeated independently twice with similar results.