Extended Data Table 1.
Cryo-EM data collection, refinement and validation statistics
| KSHV capsid (EMD-7047) (PDB 6B43) | |
|---|---|
| Data collection and processing | |
| Magnification | 14,000 |
| Voltage (kV) | 300 |
| Electron exposure (e–/Å2) | 25 |
| Defocus range (μm) | 1–3 |
| Pixel size (Å) | 1.03 |
| Symmetry imposed | Icosahedral |
| Initial particle images (no.) | 44,343 |
| Final particle images (no.) | 29,100 |
| Map resolution (Å) | 0.143 |
| FSC threshold | |
| Map resolution range (Å) | 4.2 |
| Refinement | |
| Initial model used (PDB code) | N/A |
| Model resolution (Å) | N/A |
| FSC threshold | |
| Model resolution range (Å) | N/A |
| Map sharpening B factor (Å2) | 200 |
| Model composition | |
| Non-hydrogen atoms | 214,334 |
| Protein residues | 27,223 |
| Ligands | 0 |
| B factors (Å2) | N/A |
| Protein | |
| Ligand | |
| R.m.s. deviations | |
| Bond lengths (Å) | 0.008 |
| Bond angles (°) | 1.119 |
| Validation | |
| MolProbity score | 1.98 |
| Clashscore | 7.41 |
| Poor rotamers (%) | 0.08 |
| Ramachandran plot | |
| Favored (%) | 89.26 |
| Allowed (%) | 10.59 |
| Disallowed (%) | 0.15 |