Table 1.

Fold change gene expression for high flow (HF) regions in comparison with low flow (LF) regions of the TM from anterior segments perfused at normal (1x) pressure (A) or at elevated (2x) pressure (B).

A. Genes at 1x Pressure	Fold change (HF/LF)a	B. Genes at 2x Pressure	Fold change (HF/LF)a
Collagens		Collagens
Collagen 1α1	6.14	Collagen 14α1	0.35
Collagen 6α2	2.09
Collagen 6α1	1.93
Collagen 4α2	1.76
Collagen 16α1	1.58
Collagen 15α1	0.28
ECM glycoproteins		ECM glycoproteins
Thrombospondin 2	3.4	Thrombospondin 2	1.85
SPARC	1.97	Tenascin C	0.48
Laminin β1	1.78	Laminin β1	0.42
Laminin ƴ1	0.62	Laminin α1	0.41
Laminin α3	0.49	Laminin β3	0.39
Osteopontin	0.42	ECM protein 1	0.31
Laminin β3	0.36	Osteopontin	0.20
ECM receptors/adhesion		ECM receptors/adhesion
Vascular cell adhesion molecule 1	5.22	Integrin αV	3.95
		Vascular cell adhesion molecule 1	2.17
Contactin 1	4.65	CD44	0.46
Catenin α1	4.54	Intercellular adhesion molecule 1	0.43
Catenin β1	2.49
Integrin β1	1.77
Neural cell adhesion molecule 1	1.59
Integrin β3	1.54
Integrin α7	1.50
Integrin β4	0.48
ECM regulators		ECM regulators
Matrix metalloproteinase 12	3.53	Matrix metalloproteinase 14	0.45
Matrix metalloproteinase 3	3.09	Tissue inhibitor of metalloproteinases 1	0.39
Matrix metalloproteinase 1	3.07	Matrix metalloproteinase 1	0.38
Tissue inhibitor of metalloproteinases 1	2.73	Matrix metalloproteinase 11	0.37
Matrix metalloproteinase 2	2.39	Tissue inhibitor of metalloproteinases 2	0.35
A disintegrin and metalloproteinase with thrombospondin motifs 8	0.52
Matrix metalloproteinase 16	0.44

^aThreshold fold-change differences semi-arbitrarily determined to be biologically significant were >1.5 and ≤ 0.5 as previously described (Vranka et al., 2015). SAM (significance analysis of microarrays), version 4.01 (Tusher et al., 2001), with 4 biological replicates, was used to determine statistically significant gene changes. The analysis was two class paired, with arrays median centered.