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. Author manuscript; available in PMC: 2018 Jul 10.

Published in final edited form as: Methods Enzymol. 2018 Feb 1;601:71–89. doi: 10.1016/bs.mie.2017.12.001

Fig. 3 — Time course of appearance of BFP⁺, GFP⁺, and mCherry⁺ TL cells. Above, representative example of frequency of BFP⁺ cells 0–72 h after transfection of Cas9-BFP (DSBs) and Cas9^D10A-BFP (nicks) into 293T TL cells provided with a guide RNA expression plasmid and SSO donor for HDR. Below, frequency of GFP⁺ cells (HDR) and mCherry⁺ cells (mutEJ) among BFP⁺ transfectants.