Table 1.

Typical HDR and mutEJ Frequencies

	Treatment	Donor	HDR (%)	mutEJ (%)
Nick	—	SSO	0.05–0.2	0.01–0.1
	siBRCA2	SSO	2–5	0.05–0.5
	—	dsDNA plasmid	0.2–0.5	0.01–0.1
	siBRCA2	dsDNA plasmid	0.010.1	0.3–0.8
DSB	—	SSO	2–4	1–3
	siBRCA2	SSO	2–4	1–3
	—	dsDNA plasmid	1–3	1–3
	siBRCA2	dsDNA plasmid	0.2–0.5	1–2

Frequencies were determined by scoring the fraction of GFP⁺ or mCherry⁺ cells among BFP⁺ cells in 293T TL reporter cells. As described in the text, the frequency of mutEJ is determined by a frame-shift assay, based on the fraction of mCherry⁺ cells, and the actual frequency of mutEJ is predicted to be at threefold higher than the value shown.