Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (HEK293) |
Tau RD P301S FRET Biosensor |
Produced by Diamond lab, also available from ATCC |
ATCC CRL-3275 | |
| biological sample (mouse) |
Tau KO mouse | Jackson Laboratories | 7251 | |
| biological sample (human) |
Alzheimer's Disease brain, stage VI, frozen |
Washington University in St. Louis |
62579, 62732, 61473 | |
| biological sample (human) |
Human normal brain | Washington University in St. Louis |
60974, 607, 64238 | |
| antibody | HJ8.5 | Produced by Diamond lab | N/A | Monoclonal antibody against human tau |
| antibody | Polyclonal Rabbit Anti-Human Tau |
Dako, Denmark | A0024 | |
| antibody | ECL Anti-rabbit IgG Horseradish Peroxidase F(ab) fragment |
GE Healthcare | NA9340V | |
| recombinant protein | Human tau 2N4R (Full Length WT-tau) |
Produced by Diamond lab | N/A | MAEPRQEFEV MEDHAGTYGL GDRKDQGGYT MHQDQEGDTD AGLKESPLQT PTEDGSEEPG SETSDAKSTP TAEDVTAPLV DEGAPGKQAA AQPHTEIPEG TTAEEAGIGD TPSLEDEAAG HVTQARMVSK SKDGTGSDDK KAKGADGKTK IATPRGAAPP GQKGQANATR IPAKTPPAPK TPPSSGEPPK SGDRSGYSSP GSPGTPGSRS RTPSLPTPPT REPKKVAVVR TPPKSPSSAK SRLQTAPVPM PDLKNVKSKI GSTENLKHQP GGGKVQIINK KLDLSNVQSK CGSKDNIKHV PGGGSVQIVY KPVDLSKVTS KCGSLGNIHH KPGGGQVEVK SEKLDFKDRV QSKIGSLDNI THVPGGGNKK IETHKLTFRE NAKAKTDHGA EIVYKSPVVS GDTSPRHLSN VSSTGSIDMV DSPQLATLAD EVSASLAKQG L |
| recombinant protein | Tau (2A) | Produced by Diamond lab | N/A | MAEPRQEFEV MEDHAGTYGL GDRKDQGGYT MHQDQEGDTD AGLKESPLQT PTEDGSEEPG SETSDAKSTP TAEDVTAPLV DEGAPGKQAA AQPHTEIPEG TTAEEAGIGD TPSLEDEAAG HVTQARMVSK SKDGTGSDDK KAKGADGKTK IATPRGAAPP GQKGQANATR IPAKTPPAPK TPPSSGEPPK SGDRSGYSSP GSPGTPGSRS RTPSLPTPPT REPKKVAVVR TPPKSPSSAK SRLQTAPVPM PDLKNVKSKI GSTENLKHQP GGGKVQIINK KLDLSNVQSK AGSKDNIKHV PGGGSVQIVY KPVDLSKVTS KAGSLGNIHH KPGGGQVEVK SEKLDFKDRV QSKIGSLDNI THVPGGGNKK IETHKLTFRE NAKAKTDHGA EIVYKSPVVS GDTSPRHLSN VSSTGSIDMV DSPQLATLAD EVSASLAKQG L |
| recombinant protein | Cys-Tau (2A) | Produced by Diamond lab | N/A | MAEPRQEFEV MEDHACGTYGL GDRKDQGGYT MHQDQEGDTD AGLKESPLQT PTEDGSEEPG SETSDAKSTP TAEDVTAPLV DEGAPGKQAA AQPHTEIPEG TTAEEAGIGD TPSLEDEAAG HVTQARMVSK SKDGTGSDDK KAKGADGKTK IATPRGAAPP GQKGQANATR IPAKTPPAPK TPPSSGEPPK SGDRSGYSSP GSPGTPGSRS RTPSLPTPPT REPKKVAVVR TPPKSPSSAK SRLQTAPVPM PDLKNVKSKI GSTENLKHQP GGGKVQIINK KLDLSNVQSK AGSKDNIKHV PGGGSVQIVY KPVDLSKVTS KAGSLGNIHH KPGGGQVEVK SEKLDFKDRV QSKIGSLDNI THVPGGGNKK IETHKLTFRE NAKAKTDHGA EIVYKSPVVS GDTSPRHLSN VSSTGSIDMV DSPQLATLAD EVSASLAKQG L |
| commercial assay or kit | micro BCA Protein Assay Kit | Thermo Scientific | 23235 | |
| chemical compound | Heparin sodium salt from porcine intestinal mucosa |
Sigma | H4784 |