Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (human) | HEK293T | ATCC | Cat#CRL-3216 RRID:CVCL_0063 |
|
| Cell line (human) | DLD-1 | ATCC | Cat#CCL-221 RRID:CVCL_0248 |
|
| Cell line (human) | H358 | ATCC | Cat#CRL-5807 RRID:CVCL_1559 |
|
| Transfected construct (human) | pEF-RLuc8-(GGGS)3- KRASG12D-CAAX plasmid |
This paper | N/A | DNA/protein sequences provided in the Supplementary file 1 |
| Transfected construct (human) | pEF-RLuc8-(GGGS)3- KRASG12A-CAAX plasmid |
This paper | N/A | |
| Transfected construct (human) | pEF-RLuc8-(GGGS)3- KRASG12C-CAAX plasmid |
This paper | N/A | |
| Transfected construct (human) | pEF-RLuc8-(GGGS)3- KRASG12V-CAAX plasmid |
This paper | N/A | |
| Transfected construct (human) | pEF-RLuc8-(GGGS)3- KRASG12R-CAAX plasmid |
This paper | N/A | |
| Transfected construct (human) | pEF-RLuc8-(GGGS)3- NRASQ61H-CAAX plasmid |
This paper | N/A | |
| Transfected construct (human) | pEF-RLuc8-(GGGS)3- HRASG12V-CAAX plasmid |
This paper | N/A | |
| Transfected construct (human) | pEF-RLuc8-(GGGS)3- KRASS17N-CAAX plasmid |
This paper | N/A | |
| Transfected construct (human) | pEF-RLuc8-(GGGS)3- KRASWT-CAAX plasmid |
This paper | N/A | |
| Transfected construct (human) | pEF-GFP2-(GGGS)3- CRAFS257LFL plasmid |
This paper | N/A | |
| Transfected construct (human) | pEF-PI3KαFL-(GGGS)3- GFP2 plasmid |
This paper | N/A | |
| Transfected construct (human) | pEF-CRAF RBD (aa 1–149)- (GGGS)3-GFP2 plasmid |
This paper | N/A | |
| Transfected construct (human) | pEF-PI3Kα RBD (aa 161–315)- (GGGS)3-GFP2 plasmid |
This paper | N/A | |
| Transfected construct (human) | pEF-PI3Kγ RBD (aa 190–315)- (GGGS)3-GFP2 plasmid |
This paper | N/A | DNA/protein sequences provided in the Supplementary file 1 |
| Transfected construct (human) | pEF-iDAb RAS-(GGGS)2- GFP2 plasmid |
This paper | N/A | |
| Transfected construct (human) | pEF-iDAbdm RAS-(GGGS)2- GFP2 plasmid |
This paper | N/A | |
| Transfected construct (human) | pEF-iDAb control-(GGGS)2- GFP2 plasmid |
This paper | N/A | |
| Transfected construct (human) | pEF-LMO2-(GGGS)2- RLuc8 plasmid |
This paper | N/A | |
| Transfected construct (human) | pEF-GFP2-(GGGS)3- iDAbdm LMO2 plasmid |
This paper | N/A | |
| Transfected construct (human) | pEF-memb-FLAG-iDAb RAS plasmid |
This paper | N/A | |
| Transfected construct (human) | pEF-memb-FLAG-iDAb control plasmid |
This paper | N/A | |
| Transfected construct (human) | pEF-iDAb RAS-myc plasmid |
This paper | N/A | |
| Transfected construct (human) | pEF-iDAb control-myc plasmid |
This paper | N/A | |
| Transfected construct (human) | pcDNA3.1-myc-p85αFL
plasmid |
A gift from R. Williams and O. Perisic |
N/A | |
| Transfected construct (mouse) | pEF-RALGDS RA (aa 788–884)- (GGGS)3-GFP2 plasmid |
This paper | N/A | The RALGDS RA domain corresponds to the mouse sequence |
| Antibody | Phospho-ERK 1/2 Rabbit antibody |
Cell Signaling Technology | Cat#9101S RRID:AB_331646 | |
| Antibody | Total ERK 1/2 Rabbit antibody |
Cell Signaling Technology | Cat#9102S RRID:AB_330744 | |
| Antibody | Phospho-MEK 1/2 Rabbit antibody |
Cell Signaling Technology | Cat#9154S RRID:AB_2138017 | |
| Antibody | Total MEK 1/2 Mouse antibody |
Cell Signaling Technology | Cat#4694S RRID:AB_10695868 | |
| Antibody | Phospho-AKT S473 Rabbit antibody |
Cell Signaling Technology | Cat#4058S RRID:AB_331168 | |
| Antibody | Total AKT Rabbit antibody | Cell Signaling Technology | Cat#9272S RRID:AB_329827 | |
| Antibody | Pan-RAS Mouse antibody | Millipore | Cat#OP40 RRID:AB_213400 | |
| Antibody | GFP Mouse antibody | Santa Cruz Biotechnology | Cat#sc-9996 RRID:AB_627695 | |
| Antibody | β-Actin Mouse antibody | Sigma-Aldrich | Cat#A1978 RRID:AB_476692 | |
| Antibody | CMYC HRP-linked Goat antibody |
Novus Biologicals | Cat#NB600-341 RRID:AB_10000717 |
|
| Antibody | Anti-Mouse IgG HRP-linked antibody |
Cell Signaling Technology | Cat#7076S RRID:AB_330924 | |
| Antibody | Anti-Rabbit IgG HRP-linked antibody |
Cell Signaling Technology | Cat#7074S RRID:AB_2099233 | |
| Recombinant DNA reagent | pEF-myc-cyto vector | Invitrogen | Cat#V89120 | |
| Recombinant DNA reagent | pRLuc8-N3 vector | A gift from J. Felce | Felce et al., 2017 | |
| Recombinant DNA reagent | pGFP2-N3 vector | A gift from J. Felce | Felce et al., 2017 | |
| Recombinant DNA reagent | pBABEpuro-CRAFS257L FL plasmid | Addgene | Addgene#51125 | |
| Peptide, recombinant protein | KRASQ61H | This paper | N/A | |
| Peptide, recombinant protein | KRASG12V | This paper | N/A | |
| Peptide, recombinant protein | Anti-RAS scFv | This paper | N/A | |
| Peptide, recombinant protein | Recombinant Human Epidermal Growth Factor (EGF) |
Life Technologies | Cat#PHG0311 | |
| Chemical compound, drug | Coelenterazine 400a | Cayman Chemical | Cat#16157 | |
| Chemical compound, drug | 2-bromo-6-methoxyphenol | This paper | N/A | |
| Chemical compound, drug | 3-bromobenzene-1,2-diol | This paper | N/A | |
| Chemical compound, drug | 5-bromo-2,3- dihydrobenzo[b][1,4]dioxine |
This paper | N/A | |
| Chemical compound, drug | 5-(4-chloro-3- methoxyphenyl)−2,3- dihydrobenzo[b][1,4]dioxine |
This paper | N/A | |
| Chemical compound, drug | 4-(2,3-dihydrobenzo[b] [1,4]dioxin-5-yl)-N-(4- (dimethylamino) methyl)phenyl)-2- methoxyaniline |
This paper | N/A | |
| Software, algorithm | Image J | National Institutes of Health |
https://imagej.nih.gov/ij/download.html
RRID:SCR_003070 |
|
| Software, algorithm | Prism 7.0 c | GraphPad |
https://www.graphpad.com/scientific-software/prism/
RRID:SCR_002798 |
|
| Software, algorithm | PROCHECK | Laskowski et al. (1993a) | http://www.ccp4.ac.uk/html/procheck_man/index.html | |
| Software, algorithm | REFMAC | Murshudov et al. (1997) |
http://www.ccp4.ac.uk/html/refmac5.html
RRID:SCR_014225 |
|
| Software, algorithm | MolProbity | Chen et al. (2010) |
http://molprobity.biochem.duke.edu/
RRID:SCR_014226 |
|
| Software, algorithm | Phenix | Adams et al. (2010) |
https://www.phenix-online.org/
RRID:SCR_014224 |
|
| Software, algorithm | PyMOL | Schrodinger |
https://pymol.org/2/
RRID:SCR_000305 |
|
| Other | Opti-MEM I Reduced Serum Medium, no phenol red |
Thermo-Fisher | Cat#11058021 | |
| Other | ViewPlate, White 96-well plate, clear bottom for tissue culture |
PerkinElmer | Cat#6005181 | |
| Other | BRET2 Dual Emission optical module |
PerkinElmer | Cat#2100–8140 | |
| Other | Envision instrument, Multilabel Reader |
PerkinElmer | Cat#2103 |