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. 2018 Jul 10;7:e35449. doi: 10.7554/eLife.35449

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© 2018, Wang et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 3. — (A) Schematic of in vitro transformation from cercariae to schistosomula and EdU labelling. (B) EdU⁺ cells are detected medially (1) and laterally (2L, 2R, 3L,3R) at the locations of δ-cells in cercarial bodies (ventral view). These cells divide to generate doublets, indicated by arrows. The time of EdU pulse post-transformation is indicated. Images are maximum intensity projections of confocal stacks. (C) Confocal maximum intensity projection of FISH of nanos-2 and fgfrA on schistosomula at 2 days post-transformation confirms that only δ-cells incorporated EdU. Right: magnified images of boxed cells. (D) Quantification of EdU incorporation after transformation; x axis: length of EdU treatment post-transformation. Means and standard deviations are specified. N: number of worms analyzed. (E) Irradiated cercariae exhibit no EdU⁺ cells after transformation, which confirms that EdU specifically labels proliferating cells.