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. Author manuscript; available in PMC: 2019 Jul 15.
Published in final edited form as: J Immunol. 2018 Jun 1;201(2):533–547. doi: 10.4049/jimmunol.1800250

Figure 4. gC1qR is critical for IL-12p40 suppression induced by PCV2 Cap protein.

Figure 4

(A, B) PCV2 infected wild-type (gC1qR+/+) or gC1qR knockout (gC1qR−/−) PAMs for 24 h, then the cells were stimulated by LPS/IFN-γ for 6 h or 24 h. The expression of IL-12p40 were measured by ELISA and qPCR. And the expression of gC1qR were analyzed by western blotting as the bottom panel showed. (C, D) The gC1qR+/+ and gC1qR−/− PAMs were infected by rAd-Blank, rAd-Rep, or rAd-Cap for 24 h, respectively. Then the cells were further stimulated by LPS/IFN-γ for 6 h or 24 h, and the expression of IL-12p40 were measured by ELISA and qPCR. The expression of PCV2 Rep, Cap and host gC1qR were analyzed as the bottom panel by western blotting. **P < 0.01 versus PCV2 or rAd-Cap infected gC1qR+/+ PAMs. ##P < 0.01 versus mock infection gC1qR+/+ PAMs.