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. Author manuscript; available in PMC: 2019 Jul 15.
Published in final edited form as: J Immunol. 2018 Jun 8;201(2):393–405. doi: 10.4049/jimmunol.1701588

Figure 3. TLR 7 stimulation leads to IFN-independent increases in secreting cells in the draining lymph nodes and the spleen.

Figure 3

Immune cell populations in the spleen were evaluated by flow cytometry after 2 weeks of R848 or DMSO treatment. n= 5 NZM DMSO; n= 5 NZM R848; n=5 INZM DMSO; n=5 INZM R848. A. Gating strategy for Ab-secreting cells. B, C. Graphs displaying changes in B cells: CD4−CD8−IgH+IgL+B220+ (shown in B) and Ab-secreting cells: CD4−CD8− IgH+IgLhigh (shown in C). D. Total number of cells isolated from indicated lymph nodes for DMSO or R848 treated mice. E, F. Graphs displaying changes in B cells: CD4−CD8−IgH+IgL+B220+ (shown in E) and Ab-secreting cells: CD4−CD8− IgH+IgLhigh (shown in F) for draining (cervical) and non-draining (inguinal) LN. Data is displayed as mean ± SD. Each symbol represents one mouse. LN=lymph node.