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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: Arch Insect Biochem Physiol. 2018 Apr 10;98(4):e21464. doi: 10.1002/arch.21464

FIGURE 7. Analysis of mitochondrial membrane potential in whole-mount preparations made from flies over-expressing attacin.

FIGURE 7

A. Fluorescent microscopy images of thoracic muscle and the fat body tissues stained with the JC-1 dye. Regions of high mitochondrial polarization fluoresce red due to accumulation of JC-aggregates within the mitochondria while JC-1 monomer leaks into the cytoplasm when mitochondrial membrane potential dissipates and fluoresces green. Three representative images are shown for each tissue. Preparations were made from 10 da old driver control (Da/+) and experimental flies overexpressing AttA (Da>AttA). A prominent decrease in the red/green fluorescence intensity ratio, an indicator of depolarized mitochondrial membrane, was observed in tissues of flies overexpressing AttA. The intensity of green and red fluorescence was analyzed using ImajeJ software, and the ratio of green and red fluorescence is presented in the graphs shown on the right.