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. Author manuscript; available in PMC: 2019 Jul 15.
Published in final edited form as: J Immunol. 2018 May 23;201(2):343–349. doi: 10.4049/jimmunol.1701793

Figure 4. FAM-SP-induced degranulation and binding in RBL cells overexpressing MRGPRX2.

Figure 4

(A): RBL cells stably overexpressing MRGPRX2 (RBL-MRGPRX2) were exposed to PE-conjugated anti-MRGPRX2 or PE-conjugated isotype matched antibody and cell surface receptor expression was determined by flow cytometry. (B): RBL and RBL-MRGPRX2 cells were exposed to buffer (control), SP or FAM-SP for 30 min and β-hexosaminidase release was determined. (C): RBL or RBL-MRGPRX2 cells were exposed to FAM-SP (1 μM, 60 min, 4°C), washed and fluorescence was determined by flow cytometry. Data presented for flow are representative of 3 similar experiments and degranulation data are expressed as mean ± SEM of three experiments in triplicate. Statistical significance was determined by two tailed unpaired t-Test. *** indicates P value<.001