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. Author manuscript; available in PMC: 2019 Jul 15.
Published in final edited form as: J Immunol. 2018 May 23;201(2):440–450. doi: 10.4049/jimmunol.1701654

Figure 1. PRMT1 interacts with RORγt and regulate Th17 differentiation.

Figure 1

A) PRMT1 identified by mass spectrometric analysis of RORγt-associated proteins represented in tabular form. B) Immunoprecipitation analysis of RORγt-PRMT1 interaction in Th17 cells. C) Flow cytometric analysis of the percentage of IL-17+ cells among naïve CD4+ T cells transduced with retrovirus expression GFP only (empty virus, EV) or together with PRMT1 or enzymatically inactive PRMT1-E153Q and differentiated under Th17 priming conditions for three days. D) Quantification of the results shown in C. E) Immunoblot analysis of PRMT1 expression in GFP+ cells shown in C. F) Immunoblot analysis of PRMT1 expression in CD4+ T cells transduced with retrovirus expressing indicated shRNA for knockdown PRMT1. G) Flow cytometric analysis of the percentage of IL-17+ cells among naïve CD4+ T cells transduced with retrovirus expressing indicated shRNA and differentiated under Th17 priming conditions for three days. H) Quantification of the results shown in G. *P<0.05, (D and H, One-way ANOVA, with Tukey's post-analysis multiple comparison), NS: non-significant. Error bars represent s.e.m. B, E and F are the representatives of three independent experiments.