Skip to main content
. Author manuscript; available in PMC: 2019 Jan 15.
Published in final edited form as: J Immunol. 2018 Jun 11;201(2):627–634. doi: 10.4049/jimmunol.1800515

Figure 3. IL-36 gene expression is regulated by NF-κB, MAPK and particularly PI3 kinase signaling.

Figure 3

(A) IL-36 gene expression from RNA prepared at 0, 4 and 24 h post-infection (p.i.) with C. albicans of TR146 epithelial cells pre-treated with signaling pathway inhibitors normalized to YWHAZ. Graphs depict percentage inhibition of indicated normalized gene expression relative to the DMSO vehicle control. (B) Effect of siRNA knockdown in TR146 epithelial cells on protein expression 2 h p.i. with C. albicans. (C) Impact of siRNA knockdown of c-Jun or c-Fos on gene expression of IL-36 genes 24 h p.i. with C. albicans. Graphs depict percentage inhibition of indicated normalized gene expression relative to sham transfected cells. (D) Impact of siRNA knockdown of MKP1 on gene expression of IL-36 genes 24 h p.i. with C. albicans. Graphs depict percentage inhibition of indicated normalized gene expression relative to sham transfected cells. Data are (B) representative or (A, C, D) mean + SEM of 3 independent experiments and analyzed by one-way ANOVA. *P < 0.05, **P < 0.01, and ***P < 0.001.