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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: Cold Spring Harb Protoc. 2018 Oct 1;2018(10):pdb.prot097022. doi: 10.1101/pdb.prot097022

Figure 1.

Figure 1

Co-localization of endogenous Staufen protein and injected VLE RNA. Stage II oocytes were injected with 500 nM Cy5-UTP-labeled VLE RNA (Mowry and Melton, 1992) and cultured for 18 hr to allow for vegetal localization. Immunofluorescence was performed using an anti-XStau antibody (Yoon and Mowry 2004) at 1:250 dilution and an Alexa-546-secondary antibody (Thermo-Fisher) at 1:500 dilution. Oocytes were imaged on a Zeiss LSM 510 Meta Confocal Laser Scanning Microscope using a 20× objective. Shown is a confocal section with (A) VLE RNA in red, (B) Staufen protein in green, and (C) merged red and green channels, showing Staufen protein and VLE RNA colocalization. The oocyte is oriented with the vegetal pole at the bottom and the scale bars = 50 µm.