Fig. 2.

Genome-wide distribution of drug-enriched loci in Cos-Seq amastigote selection. (A)L. infantum 36-chromosome map of genes significantly enriched by antimony (Sb^V), miltefosine (MF) and amphotericin B (AMB) as revealed by Cos-Seq. Gray bars represent gene positions on each chromosome. Colored bars represent drug-enriched genes according to the color code shown below. Only genes enriched with a mean log2-fold change of ≥3 are depicted. Asterisks denote the genomic loci enriched by Cos-Seq that were recovered and characterized for drug resistance. (B) Highly enriched (log2-fold change of ≥4) cosmids retrieved from drug selections normalized to the drug-free control. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)