Fig. 5.

Ezrin knockdown inhibits fibroblasts proliferation via impaired YAP activity. Cells were transfected with non-specific control siRNA or Ezrin siRNAs or Ezrin siRNAs plus constitutively active YAP for two days. a Cells were harvested two days after transfection and 2 × 10⁵ cells were cultured in 60 mm plates for one day (top panel) and 6 days (bottom panel) followed by stain with crystal violet blue dye to visualize cells. N = 4. b To quantify crystal violet, methanol was added to solubilize the dye, followed by reading optical density (O.D.540). Results are expressed as the mean ± SEM, N = 4, *p < 0.05 vs control si. c Cells were harvested two days after transfection and 2.5 × 10⁵ cells were cultured in 60 mm plates. Cells harvested at indicated days and counted. Data are expressed as mean±SEM. N = 4