Fig. 4.
Number of copies obtained from the quantitative PCR at IC50 and IC100 and non-treated control well (parasites grown without the addition of Draxxin®, represented as “0”). A) B. bovis, B) B. bigemina and C) T. equi. On the top of each bar is presented the PPE quantified. Assay was carried out in triplicate, and samples analyzed individually after 72 h in culture. Error bars indicate the standard error of the means for each sample analyzed. (*) Represents p-value <0.05 indicating a statistically significant difference between media and correspondent Draxxin® using one-way ANOVA.