Table 1.
The primers and cycling conditions used to amplify Pvdhfr, Pvdhps, Pvmdr1, Pvcrt-o and Pvk12 of P. vivax isolates.
| Genes | Sequences (5’ → 3′) | PCR cycling conditions | Product size (bp) | Reference |
|---|---|---|---|---|
| Pvdhfr (1st) | F: CACCGCACCAGTTGATTCCT | 94 °C 5 min/[94 °C 30 s, 58 °C 30 s, 68 °C 1 min] × 20 cycles, 68 °C 5 min | 979 | Ding et al., 2013 |
| R: CCTCGGCGTTGTTCTTCT | ||||
| Pvdhfr (2nd) | F: CCCCACCACATAACGAAG | 94 °C 5 min/[94 °C 30 s, 58 °C 30 s, 68 °C 45 s] × 40 cycles, 68 °C 5 min | 755 | |
| R: CCCCACCTTGCTGTAAACC | ||||
| Pvdhps (1st) | F: GATGGCGGTTTATTTGTCG | 94 °C 5 min/[94 °C 30 s, 59 °C 30 s, 68 °C 1 min] × 20 cycles, 68 °C 5 min | 1009 | |
| R: GCTGATCTTTGTCTTGACG | ||||
| Pvdhps (2nd) | F: GCTGTGGAGAGGATGTTC | 94 °C 5 min/[94 °C 30 s, 59 °C 30 s, 68 °C 45 s] × 40 cycles, 68 °C 5 min | 731 | |
| R: CCGCTCATCAGTCTGCAC | ||||
| Pvmdr1 (1st) | F: ACGACATGATCCAAACGACA | 94 °C 5 min/[94 °C 30 s, 60 °C 30 s, 68 °C 3 min] × 20 cycles, 68 °C 5 min | 2784 | Barnadas et al., 2011 |
| R: CTTATATACGCCGTCCTGCAC | ||||
| Pvmdr1 (2nd) | F: GGATAGTCATGCCCCAGGATTG | 94 °C 5 min/[94 °C 30 s, 62 °C 1 min, 68 °C 45 s] × 40 cycles, 68 °C 5 min | 604 | Lu et al., 2011 |
| R: CATCAACTTCCCGGCGTAGC | ||||
| Pvcrt-o | F: AAGAGCCGTCTAGCCATCC | 94 °C 5 min/[94 °C 30 s, 62 °C 1 min, 68 °C 1.30 min] × 40 cycles, 68 °C 5 min | 1186 | Lu et al., 2012 |
| R: AGTTTCCCTCTACACCCG | ||||
| Pvk12 (1st) | F: ATCCAACAGCATTTCCAACT | 94 °C 15 min/[94 °C 30 s, 58 °C 1 min,682 °C 2.10 min] × 20 cycles, 68 °C 10 min | 2108 | Popovici et al., 2015 |
| R: CAATTAAAACGGAATGTCCA | ||||
| Pvk12 (2nd) | F: ACCACGTGACGAGGGATAAG | 94 °C 15 min/[94 °C 30 s, 62 °C 1 min, 68 °C 1.30 min] × 20 cycles, 68 °C 10 min | 1015 | |
| R: AAAACGGAATGTCCAAATCG |