Fig. 4.
Whole cell assays with WBC6 (WT; white symbols) and nitro drug resistant (C4; black symbols) trophozoites. A, oxygen consumption (OCR) and extracellular acidification (ECAR) rates determined in a Seahorse XFp device. Mean values (±) SE for three independent assays are given (*, p < 0.01). B, resazurin reduction. Mean values (+SE) from quintuplicate determinations in 96-well-plates (104 trophozoites per well) are given. For both strains, the rates were determined in absence (circles) and presence (triangles) of glucose. Both assays were performed as described in Materials and Methods.