Table 1.
Primers used in this study. Gene annotations and accession numbers were retrieved from GiardiaDB (giardiadb.org). The genes marked with an asterisk are lateral transfer candidates.
| Name | Sequence | GiadiiaDB accession number |
|---|---|---|
| ACT_F | ACATATGAGCTGCCAGATGG | Actin related protein (GL50803_40817) |
| ACT_R | TCGGGGAGGCCTGCAAAC | |
| FDP_F | TGGGTGGAGCAACAGGGC | A-type flavoprotein; flavodiiron* protein (GL50803_10358) |
| FDP_R | TTACTGCTTAGGGGCGTTCT | |
| FlaHB_F | GGACAGAGAGGGCGAGGA | Flavohemoprotein* (GL50803_15009) |
| FlaHB_R | CTAATGGGAGGCCTTGAAG | |
| NO_F | GCACGACACGCATCATCC | NADH oxidase |
| NO_R | TTACAGTTTCATCAGCGTGG | (GL50803_9719) |
| NOLT_F | ACACGGACAGGCCTGGGT | NADH oxidase lateral transfer |
| NOLT_R | TCAGTCCTTCTTGTTTATCGCAC | candidate* (GL50803_33769) |
| GlNR1_F | CCTGCTGACAAGGCCGCA | Nitroreductase Fd-NR2* (GL50803_22677) |
| GlNR1_R | AACACCAATTACTTAAATGTAATG | |
| GlNR2_F | CTGCAGCTTCACTCAGAGA | Nitroreductase Fd-NR1* (GL50803_6175) |
| GlNR2_R | TTATTCCACAAACGTTACGTC | |
| NRfam_F | GGGAATACAAAATGACGGGG | Nitroreductase family protein* (GL50803_15307) |
| NRfam_R | GTACTCTTCTGTTTGGCGAG | |
| POR1_F | ATCCAACGCGACCCAGAAG | Pyruvate-flavodoxin-oxido-reductase (GL50803_17063) |
| POR1_R | GTTCACTGCTTACTCCGCC | |
| POR2_F | CTCGCACATGGTCCAGGG | Pyruvate-flavodoxin-oxido-reductase (GL50803_114609) |
| POR2_R | AGAGCCGCAGCCATCTCC | |
| TrxR_F | CGTTGGCCACGATCCCC | Thioredoxinreductase (GL50803_9827) |
| TrxR_R | TACTCCTGCATGGCAAGCC |