Table 3.
Expression of G. lamblia enzymes involved in reduction of nitro compounds in the MET/NTZ resistant strain C4 as compared to the wild-type (WT) WBC6. Trophozoites were grown to confluence and harvested as described. To determine mRNA levels, RNA was extracted and reverse transcribed into cDNA. Transcripts were quantified in relation to actin. Mean values ± SE are given for four independent samples. Values marked by asterisks are significantly different to the control (paired t-test, two-sided; *, P < 0.001). The full names of the genes, the accession numbers and the primers are given in Table 1 n.d., not detected.
| Gene | mRNA levels |
|
|---|---|---|
| WT | C4 | |
| FDP | 1.9 ± 0.0 | 1.8 ± 0.1 |
| FlaHb | 1.1 ± 0.01 | 0.1 ± 0.01 |
| NR1 | 0.18 ± 0.03 | 0.03 ± 0.01* |
| NR2 | 0.13 ± 0.01 | 0.16 ± 0.02 |
| NRfam | 0.05 ± 0.01 | 0.07 ± 0.003 |
| POR1 | 1.5 ± 0.2 | 2.1 ± 0.2 |
| POR2 | 3.1 ± 0.4 | 5.7 ± 0.01 |
| TrxR | 4.4 ± 0.01 | 4.6 ± 0.3 |
| NO | 8.4 ± 0.5 | 8.3 ± 0.5 |
| NOLT | 8.8 ± 0.7 | 14.7 ± 0.6 |