Table 2.

Trypanocidal concentrations of AS-48 do not permeabilize the parasite plasma membrane. The effect of AS-48 on plasma membrane permeability was determined by flow cytometry using SYTOX^® Green as described in Materials and Methods. Intracellular fluorescence after 0.1% Triton™ X-100 treatment was used as a control of 100% permeabilization. Parasite death under the same conditions was monitored using optical microscopy to calculate the percentage of non-motile parasites.

AS-48 (μM)	Time (min)	4 °C		37 °C
		Intracellular fluorescence (% of control)	Cell death (%)	Intracellular fluorescence (% of control)	Cell death (%)
0.05	10	<2	<10	<2	12.0 ± 3.4
	30	<2	<10	<2	26.2 ± 20.8
	60	<2	<10	<2	34.6 ± 4.8
0.1	10	<2	<10	<2	9.4 ± 3.4
	30	<2	<10	<2	37.6 ± 26.0
	60	<2	<10	<2	63.4 ± 12.5
0.5	10	<2	<10	<2	26.6 ± 13.9
	30	<2	<10	3.3 ± 3.1	86.0 ± 12.4
	60	<2	<10	7.1 ± 0.5	95.5 ± 2.5