Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jul 10;9:2668. doi: 10.1038/s41467-018-05127-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — Screen of small molecules for SOD1-Derlin-1 interaction inhibitors. a TR-FRET-based interaction assay model showing the case of tagged SOD1 as a target of the Eu-labeled antibody and tagged Derlin-1 as a target of the d2-labeled antibody. b, c FRET signals with Flag-Eu and HA-d2 shown as fold changes from the FRET signal in non-transfected lysate. The data are shown as the mean ± s.d. b FRET signal using lysates from HEK293A cells transfected with plasmids as indicated (n = 4). c Competition assay of FRET signal generated by Flag-SOD1^G93A and Derlin-1-HA using indicated concentration of SOD1 (5-20) and Derlin-1 (CT4) peptide. Lysates from HEK293A cells transfected with Flag-SOD1^G93A and Derlin-1-HA were incubated with the indicated concentration of each peptide for 16 h (n = 3). Light gray: with Derlin-1 (CT4) peptide; dark gray: with SOD1 (5-20) peptide. d Result of the first screening. The compounds are presented in order of inhibition rate. e, f Blue: inhibition (%) against FRET signal generated by Flag-SOD1^G93A and Derlin-1-HA; red: inhibition (%) against FRET signal generated by HA-SOD1^WT-Flag. e Result of the second screening. The compounds are presented in order of inhibition rate against the SOD1-Derlin-1-derived FRET signal. f Result of #56 in the third screening. The data are shown as the mean ± s.d. (n = 4). g Chemical structure of #56