Table 3.
Screen specific modifications of applied filters before precursor selection.
| Screen | Description | Intensity Filter | Charge Filter | DE | Apex Detection |
|---|---|---|---|---|---|
| I. | (Intensity) | 1e5 | N/A | N/A | N/A |
| II. | (Anion) | 1e5 | −1 | N/A | N/A |
| III. | (Dianion) | 1e5 | −2 | N/A | N/A |
| IV. | (DE 12 s) | 1e5 | N/A | t = 12 s | N/A |
| V. | (DE 3 s) | 1e5 | N/A | t = 3 s | N/A |
| VI. | (DE 3 s, Apex) | 1e5 | N/A | t = 3 s | 6 s, 30% |
| VII. | (−1, DE 3 s) | 1e5 | −1 | t = 3 s | N/A |
| VIII. | (−2, DE 3 s) | 1e5 | −2 | t = 3 s | N/A |
| IX. | (DE 3 s, 5e5) | 5e5 | N/A | t = 3 s | N/A |
| X. | (−1, DE 3 s, 5e5) | 5e5 | −1 | t = 3 s | N/A |
| XI. | (−2, DE 3 s, 5e5) | 5e5 | −2 | t = 3 s | N/A |
Common settings between methods included: OT-MS Scan: 50 K; MIPS Filter: Peptide; 5 ddMS2 OT-HCD Scans: HCD CE 40 +/− 5, 15 K; TRT: T-3RT or T-2RT as stated; 1 ddMS2 OT-CID Scan: CE 30, 15 K.