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. 2018 Jul 10;8:10410. doi: 10.1038/s41598-018-28506-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Perturbation of the EHT by the bromodomain inhibitor JQ1. (A) Representative flow cytometry analysis from four independent experiments showing the effect of treatment with JQ1 on the differentiating cell populations. Adherent cells from each of the treatment conditions were stained with c-Kit-APC, CD41-PECy7 and Tie2-PE. Populations were gated on c-Kit positive cells (upper histogram) and cells within this gate expressing CD41 and Tie2 are shown in the quadrant plot below. The bottom left panel indicates the cell populations represented in each of the quadrants of cells stained with c-Kit-APC, CD41-PECy7 and Tie2-PE and also indicates the direction of differentiation. (B) Heatmap showing hierarchical clustering of log2 FPKM for the 2124 genes that change expression at least 2 fold between treatment conditions as defined in Supplementary Fig. 2B. (C) Changes in gene expression were grouped according to whether genes were up-regulated (1), down-regulated (0) or unchanged (2) between the indicated treatment conditions. The number of the group relates to changes between the +Dox compared to the No Dox sample (1^st column) and changes between the +Dox +JQ1 compared to the +Dox sample (2^nd column). GO terms for selected groups are indicated on the right hand side of the figure. See related Figure S2D for numbers of genes within each group. (D) Average profile of H3K79me2 enrichment for each of the 8 gene expression groups shown in Fig. 2C.