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. 2018 Jul 10;9(7):767. doi: 10.1038/s41419-018-0799-z

Fig. 4. Comp 5 induces mitophagy via SIRT1–PINK1–Parkin pathway in GBM cells.

Fig. 4

a U87MG and T98G cells were treated with 10 μM Comp 5 for 24 h, then fixed with 90% ethanol and incubated with PINK1 primary antibody overnight. After incubated with fluorescence-secondary antibody for 2 h, cells were stained with DAPI for 20 min and observed under fluorescence microscope. Scale bar = 10 μm. b U87MG and T98G cells were treated with 10 μM Comp 5 for 24 h, then the expression levels of PINK1, Parkin, BNIP3, VDAC1, ATG5, ATG12-ATG5, and ATG16L were determined by western blot. β-actin was used as a loading control. c U87MG and T98G cells were transfected with SIRT1 siRNA for indicated time and treated with or without Comp 5 for additional 24 h. The expression level of PINK1 was determined by western blot. β-actin was used as a loading control