Fig. 2. RNA-seq reveals distinct expression pattern of miRNAs, lncRNAs, and mRNAs in human primary OS, pulmonary metastatic OS, and normal controls.

a–c Principal component analyses of miRNA, lncRNA, and mRNA expression profiles. d–f Unsupervised hierarchical clustering of the expression profiles of DEmiRNAs, DElncRNAs, and DEmRNAs. g Circus plot representing the distribution of DElncRNAs and DEmRNAs in primary OS vs. normal controls on chromosomes. h–j Unsupervised clustering analysis showing expression profiles of DEmiRNAs, DEmRNAs, and DElncRNAs in primary OS vs. normal controls. k Circus plot representing the distribution of DElncRNAs and DEmRNAs in pulmonary metastatic OS vs. primary OS on chromosomes. l–n Unsupervised clustering analysis showing expression profiles of DEmiRNAs, DEmRNAs, and DElncRNAs in pulmonary metastatic OS vs. primary OS