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. 2018 Jul 10;9(7):773. doi: 10.1038/s41419-018-0806-4

Fig. 5. Aggressive and drug-resistant tumor cell lines are more susceptible to 26S depletion.

Fig. 5

A panel of various cell lines harboring doxycycline-inducible PSMD1 shRNA were treated with doxycycline to induce PSMD1 shRNA expression or left untreated. Alternatively, cells were transfected with siRNA against PSMD1 or luciferase gene. Cell proliferation was quantified as described in Fig. 3e. a Heat map analysis showing the relative survival of multiple cell lines in the course of 26S depletion (left). Heat map analysis showing relative survival of an isogenic cell line pair (MCF10A versus Ras-transformed counterpart) (right). Relative survival is calculated as a ratio of viability of PSMD1 shRNA-expressing cells to the viability of control cells measured at the same time point. b Relative survival of different cell lines measured at day 5 of induction. Mutational status of the p53 gene is depicted for each cell line. c Relative survival at day 5 plotted against the doubling time of the tested cell lines. d Relative survival at day 5 plotted against PSMD1 KD efficiency in the tested cell lines (PSMD1 KD efficiency is calculated as a ratio of remaining PSMD1 levels in shRNA-induced cells divided by the PSMD1 levels of control cells at the same time point)