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. 2018 Jul 10;8:10415. doi: 10.1038/s41598-018-28816-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of insulin on c-Fos expression in the hypothalamus. (A) Representative pictures showing c-Fos expression in the PVN, ARC and VMH of hypothalamus at 90 min after ICV injection of saline or insulin (100 μU/2 μl). Scale bar: 200 μm. (B) Number of c-Fos- immunopositive (IR) neurons per section of the hypothalamic nuclei at 90 min after ICV injection of saline (white bars) or insulin (100 μU/2 μl) (black bars). n = 5. **p < 0.01 and *p < 0.05 vs. saline by one-way ANOVA followed by Tukey’s test. Error bars are SEM.