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. 2018 Jul 10;8:10415. doi: 10.1038/s41598-018-28816-w

Figure 8.

Figure 8

Insulin increases [Ca2+]i in smaller-sized oxytocin neurons of PVN. (A,B) Representative [Ca2+]i responses to 10−11 M insulin in smaller-sized oxytocin neurons of PVN, possibly representing parvocellular neurons, isolated from Oxy Cre mice (A) and Oxy Pdk1 KO mice (B). Single neurons with diameter <25.0 μm were classified as smaller-sized neurons in this study. (C) Incidence of [Ca2+]i responses to insulin in PVN oxytocin neurons, expressed by percentage. Numbers around each point indicate the number of PVN oxy neurons. (D) Average amplitude of [Ca2+]i responses to insulin (Δratio) in oxytocin neurons examined. Scale bar: 25 μm.