Skip to main content
. 2018 Jul 10;9(7):771. doi: 10.1038/s41419-018-0795-3

Fig. 3. Cav-1 was degraded by PA-induced autophagy.

Fig. 3

a Real-time qPCR showing the expression of Cav-1 mRNA in the astrocyte exposed to PA (0.25 mM, 12 h) (mean ± S.E.M. n = 3). b The representative immunofluorescence images of Cav-1 in the astrocytes exposed to PA (0.25 mM, 12 h). Cav-1 was shown in red; GFAP was shown in green. Scale bar, 100 μm, n = 3. Western blots showing the expression of Cav-1 in the astrocytes incubated with PA (0.25 mM, 12 h) in the absence and presence of 3-MA (c) or CQ (c) or 10 μmol/L MG132 (d) (mean ± S.E.M. n = 3, *P < 0.05 vs. BSA; #P < 0.05 vs. PA). e Western blots showing the expression of Cav-1 in ATG5 or ATG7-deficient astrocytes exposed to PA (0.25 mM, 12 h) (mean ± S.E.M. n = 3, *P < 0.05 vs. BSA + SC-siRNA; #P < 0.05 vs. PA + SC-siRNA). f Western blots showing the expression of LC3B-II in Cav-1-deficient astrocytes exposed to PA (0.25 mM, 12 h) (mean ± S.E.M. n = 3, *P < 0.05 vs. BSA + SC-siRNA). g Western blots showing the expression of LC3B-II in Cav-1 overexpressing astrocytes exposed to PA (0.25 mM, 12 h) (mean ± S.E.M. n = 3, *P < 0.05 vs. BSA + Ad-null)