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. 2018 Jul 10;9(7):777. doi: 10.1038/s41419-018-0778-4

Fig. 4. PAI-1 was a target of miR-34a and could be involved in the regulation of OS dedifferentiation.

Fig. 4

a, b Both mRNA (a) and protein (b) expression levels of PAI-1 were upregulated during the dedifferentiation of MNNG/HOS and MG-63 cells. c, d Inhibition of PAI-1 by PAI-039 treatment (c) or siRNA transfection (d) could suppress the forming of sarcospheres. Scale bar = 100 μm. e qPCR analysis of PAI-1 mRNA expression in MNNG/HOS and MG-63 cells after transfected with miR-34a-5p mimic or mimic NC. f Immunoblotting of PAI-1 in five groups of MNNG/HOS and MG-63 cells (mock, inhibitor NC, miR-34a-5p inhibitor, mimic NC and miR-34a-5p mimic). g Overexpression of miR-34a-5p significantly suppressed the luciferase activity of the PAI-1 WT 3′-UTR luciferase reporter construct but had no effect on the mutant construct. *P < 0.05, **P < 0.01, #P > 0.05