Fig. 5. Inhibition of PAI-1 suppressed OS dedifferentiation via Sox2 downregulation.

a The mRNA levels of Sox2 were downregulated after miR-34a overexpression in OS cells. b–e The inhibition of PAI-1 by PAI-039 (b, d) or siRNAs (c, e)-mediated gene silencing could downregulate the mRNA (b, c) and protein (d, e) levels of Sox2 in both MNNG/HOS and MG-63 cells. f, g PAI-039 could reduce both mRNA (f) and protein (g) expression level of Sox2 promoted by miR-34a downregulation. h, i Representative images showing that Sox2 overexpression could rescue the suppression of MNNG/HOS (h) and MG-63 (i) dedifferentiation caused by PAI-1 inhibition. Scale bar = 100 μm. *P < 0.05, **P < 0.01