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. 2018 Jul 10;8:10376. doi: 10.1038/s41598-018-28415-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Acute alcohol challenge in adulthood epigenetically normalizes Creb1, Cbp, and p300 mRNA expression in the amygdala of rats exposed to adolescent intermittent ethanol (AIE). (a) Bar diagram showing fold changes in mRNA levels of Creb1, Cbp, and p300 in the amygdala of adolescent intermittent saline (AIS) and AIE rats exposed to an acute challenge of ethanol or saline in adulthood. Occupancy of acetylated histone 3 lysine 9 and 14 (H3K9/14ac) protein was investigated using the chromatin immunoprecipitation (ChIP) assay and primers specific for two sites in the promoter regions of Creb1 (b) Cbp (c) and p300 (d) in the amygdala of AIS and AIE adult rats exposed to an acute challenge of ethanol or saline. Values are presented as the mean ± SEM of the fold changes relative to AIS + Saline control rats. *p < 0.05, **p < 0.01, ***p < 0.001, two-way ANOVA followed by Tukey’s post hoc test, (n = 8–9/group for mRNA studies and n = 5–6/group for ChIP assay). CRE = cAMP response element; bp = base pairs.