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. 2018 Jul 10;8:10414. doi: 10.1038/s41598-018-28682-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The GABA_B receptor forms high order oligomers in cultured hippocampal neurons. (a) Schematic representation of the mGlu₂ receptor and the three combinations of untagged and SNAP-tagged GABA_B receptor subunits. (b-c) Comparison of the subunits per complex for all GABA_B receptor combinations (grey) with the mGlu₂ receptor (black) as a function of the expression level (b) or for cells with an expression in the 90–600 subunits/V_eff range (c). The number of subunits per complex is higher for the GABA_B receptor than for the mGlu₂ receptor (p < 0.0001; unpaired, two-sided t-test). Error bars represent SD. Data are from 14–17 independent experiments.