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. 2018 Jul 10;8:10403. doi: 10.1038/s41598-018-28427-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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COXIV-1 and COXIV-2 expression, and COX activity in PC12 cell derived neuron-like cells. Neuron-like cells were induced from rat pheochromocytoma (PC12) cells, by 5 ng/mL nerve growth factor. Then, neuron-like cells were exposed to 30 mW/cm² microwaves for 6 min. Cells were harvested at various time points after exposure. Protein expression of COXIV-1 (A) and COXIV-2 (B) were analyzed by western-blotting. And, the ratio of COXIV-1 to COXIV-2 was presented in (C). Moreover, The COX activity was detected by COX assay kit and normalized by protein concentration (D). Data were shown as mean ± s.e.m. *p < 0.05, **p < 0.01 compared to corresponding group.