Figure 4.

Microwave exposure promotes HIF-1α nuclear translocation in neuron-like cells. Neuron-like cells were exposed to 30 mW/cm² microwave. After exposure, the total HIF-1α protein expression was detected by western-blotting, and normalized by GAPDH (A). Moreover, nuclear protein was also extracted. Then, nuclear HIF-1α level was detected by western-blotting, and normalized by Lamin B (B). Immunofluorescence staining was conducted to show the expression and distribution of HIF-1α. The representative images were captured under fluorescent microscope (C). Data were shown as mean ± s.e.m. *p < 0.05, **p < 0.01 vs corresponding group.