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. 2018 Jul 10;8:10394. doi: 10.1038/s41598-018-28500-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Translocation of NF-κB subunits from cytoplasm to nucleus upon BaP exposure. U1 cells were treated with BaP 100 nm (A) and 1 µM (B) for 7 days and 3 days, respectively. After the treatment, proteins from the cytoplasm and nucleus were extracted from the cells. Western blot was run to determine the expression of the NF-κB p50 and p65 subunits in the proteins in cytosolic fraction (CF) and nuclear fraction (NF). GAPDH and β-lamin were used as loading controls for the cytoplasmic and nuclear proteins, respectively. The blots are representative of at least three independent experiments. There is not much difference in the expression of NF-κB p50 and p65 between the control and the BaP-treated cells in the cytoplasmic fraction. However, there is a clear increase in the expression of both the subunits in the nuclear fraction of acutely or chronically BaP-treated cells compared to the control group.