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. 2018 Jul 10;8:10385. doi: 10.1038/s41598-018-28502-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Identification of the bRosa26 locus. (A) Diagram of the bRosa26 locus on chromosome 22. (B) The expression of the bRosa26 lncRNA in various tissues relative to GAPDH was determined using Q-PCR. Error bars represent the mean ± SD. (C) The expression of the bRosa26 lncRNA in various tissues relative to GAPDH was analyzed using RT-PCR. For RT-PCR, the designed primers annealed to the bRosa26 sequence and amplified a correctly spliced product of 155 bp. GAPDH served as a control (488 bp).