Figure 1.

Donor-specific antibodies (DSAs) induced by skin graft sensitization were affected by donor-recipient combination and skin graft size and were sustained for a long time. Skin grafts of size 8–10 × 10 mm² were transplanted onto the back of recipient mice. Recipient sera collected at the indicated time points were reacted with donor spleen cells and evaluated by immunofluorescence and flow cytometry. Levels of detected IgG and IgM antibodies are expressed as mean fluorescence intensity (MFI) ± SD (n = 6). (A) C3H-Balb/c. (B) C57BL/6-Balb/c, skin grafts of size 4–5 × 5 mm² were also applied for comparison with the grafts measuring 8–10 × 10 mm². (C) Balb/c-C3H. (D) Balb/c-C57BL/6.